Evaluation of the feasibility of using naringinase to obtain hesperetin 7-O-glucoside
Glycosylation naturally affects the biophysical and biochemical properties of flavonoids, as well as their biological activity. The presence of sugar groupings can lead to a change in the bioavailability of the corresponding flavonoid aglycone depending on the nature of the sugar, as glucosides, for example, are absorbed faster than other types of glycosides, such as rhamnosides and rhamnoglucosides. Due to the hindered absorption of flavonoids, solutions seeking to increase their bioavailability are known, one of which is their deglycosylation.
Naringinase is an enzyme complex that exhibits dual activity: α-L-rhamnosidase and β-D-glucosidase. Among other things, this enzyme can hydrolyze hesperidin, a flavonoid commonly found in citrus fruits, primarily oranges. Hesperidin can be deglycosylated by α-rhamnosidase to rhamnose and hesperetin 7-O-glucoside (Hes-7-G), which is then hydrolyzed by β-D-glucosidase to glucose and hesperetin. Hes-7-G is better soluble in water than hesperidin and its aglycone, hesperetin. Enzymatic bioconversion of hesperidin to Hes-7-G may change the site of absorption and increase its bioavailability in juices, which is desirable for practical applications in the food industry.
The aim of the project is to test the feasibility of using naringinase from Aspergillus niger KMS to efficiently hydrolyze a preparation of hesperidin and this flavonoid contained in orange juice to obtain hesperetin 7-O-glucoside (Hes-7-G). For targeted hydrolysis leading to Hes-7-G, it will be necessary to deactivate β-D-glucosidase.
Achieving this goal will require:
I. Determination of optimal conditions for hydrolysis of hesperidin by naringinase to obtain Hes-7-G.
II. Determination of β-D-glucosidase deactivation conditions for targeted hydrolysis leading to Hes-7-G.
III. Characterize naringinase and its subunits in the hydrolysis reaction of hesperidin under conditions leading to the formation of Hes-7-G.
National Science Center
Programme:
MINIATURA 8
Project duration:
08/08/2024 – 07/08/2025
Funding amount:
31 350 PLN
Project status:
Project in progress
